statistical analysis v8 sas 2000 Search Results


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Organ and tissue specificity of DEG10 promoter activity in Arabidopsis. Histochemical GUS staining was performed on T1 (A–F) and T2 (G, H) transgenic plants carrying either a Pr DEG10 :GFP:GUS (B, D, G, H) or a Pr DEG10 :GUS (A, C, E, F) expression cassette. (A–D) Different patterns of GUS expression observed in leaves of 6-week-old plants cultivated on soil in a glasshouse. (E, F) GUS expression in reproductive organs of approximately 3-month-old plants, showing GUS activity in pollen and at the stigma. Arrows in E indicate very young buds, in which no GUS activity was detected. Arrows in F indicate staining in the stigma and in pollen grains shattered from the anthers and caught in the base of the flower. (G, H) Staining of 18-day-old, whole T2 seedlings. Images were taken with a <t>stereomicroscope.</t>
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Organ and tissue specificity of DEG10 promoter activity in Arabidopsis. Histochemical GUS staining was performed on T1 (A–F) and T2 (G, H) transgenic plants carrying either a Pr DEG10 :GFP:GUS (B, D, G, H) or a Pr DEG10 :GUS (A, C, E, F) expression cassette. (A–D) Different patterns of GUS expression observed in leaves of 6-week-old plants cultivated on soil in a glasshouse. (E, F) GUS expression in reproductive organs of approximately 3-month-old plants, showing GUS activity in pollen and at the stigma. Arrows in E indicate very young buds, in which no GUS activity was detected. Arrows in F indicate staining in the stigma and in pollen grains shattered from the anthers and caught in the base of the flower. (G, H) Staining of 18-day-old, whole T2 seedlings. Images were taken with a <t>stereomicroscope.</t>
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Organ and tissue specificity of DEG10 promoter activity in Arabidopsis. Histochemical GUS staining was performed on T1 (A–F) and T2 (G, H) transgenic plants carrying either a Pr DEG10 :GFP:GUS (B, D, G, H) or a Pr DEG10 :GUS (A, C, E, F) expression cassette. (A–D) Different patterns of GUS expression observed in leaves of 6-week-old plants cultivated on soil in a glasshouse. (E, F) GUS expression in reproductive organs of approximately 3-month-old plants, showing GUS activity in pollen and at the stigma. Arrows in E indicate very young buds, in which no GUS activity was detected. Arrows in F indicate staining in the stigma and in pollen grains shattered from the anthers and caught in the base of the flower. (G, H) Staining of 18-day-old, whole T2 seedlings. Images were taken with a <t>stereomicroscope.</t>
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(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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Carl Zeiss stereoscopic light microscope
(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), <t>V8</t> <t>proteinase</t> (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)
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Image Search Results


Organ and tissue specificity of DEG10 promoter activity in Arabidopsis. Histochemical GUS staining was performed on T1 (A–F) and T2 (G, H) transgenic plants carrying either a Pr DEG10 :GFP:GUS (B, D, G, H) or a Pr DEG10 :GUS (A, C, E, F) expression cassette. (A–D) Different patterns of GUS expression observed in leaves of 6-week-old plants cultivated on soil in a glasshouse. (E, F) GUS expression in reproductive organs of approximately 3-month-old plants, showing GUS activity in pollen and at the stigma. Arrows in E indicate very young buds, in which no GUS activity was detected. Arrows in F indicate staining in the stigma and in pollen grains shattered from the anthers and caught in the base of the flower. (G, H) Staining of 18-day-old, whole T2 seedlings. Images were taken with a stereomicroscope.

Journal: Journal of Experimental Botany

Article Title: DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis

doi: 10.1093/jxb/erz294

Figure Lengend Snippet: Organ and tissue specificity of DEG10 promoter activity in Arabidopsis. Histochemical GUS staining was performed on T1 (A–F) and T2 (G, H) transgenic plants carrying either a Pr DEG10 :GFP:GUS (B, D, G, H) or a Pr DEG10 :GUS (A, C, E, F) expression cassette. (A–D) Different patterns of GUS expression observed in leaves of 6-week-old plants cultivated on soil in a glasshouse. (E, F) GUS expression in reproductive organs of approximately 3-month-old plants, showing GUS activity in pollen and at the stigma. Arrows in E indicate very young buds, in which no GUS activity was detected. Arrows in F indicate staining in the stigma and in pollen grains shattered from the anthers and caught in the base of the flower. (G, H) Staining of 18-day-old, whole T2 seedlings. Images were taken with a stereomicroscope.

Article Snippet: Photographs were taken with a stereomicroscope (Stemi 2000-C, Zeiss) with an external light source (KL 1500 LCD, Zeiss).

Techniques: Activity Assay, Staining, Transgenic Assay, Expressing

(A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), V8 proteinase (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)

Journal: PLoS ONE

Article Title: End-Tagging of Ultra-Short Antimicrobial Peptides by W/F Stretches to Facilitate Bacterial Killing

doi: 10.1371/journal.pone.0005285

Figure Lengend Snippet: (A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), V8 proteinase (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In , the difference between tagged and non-tagged peptide is statistically significant in all cases (P<0.002, one way ANOVA).)

Article Snippet: Peptides (1 μg) were incubated at 37°C with S. aureus aureolysin (0.1 μg, 25000 units/mg), S. aureus V8 proteinase (0.1 μg, 2000 mU), both from BioCol GmbH (Potsdam, Germany), or neutrophil elastase (0.4 μg, 29 units/mg; Calbiochem (La Jolla, USA)) in a total volume of 30 μl for 3 hours.

Techniques: Incubation, SDS Page, Ex Vivo, Infection, Bacteria